Dire Wolves have become more known as mythical symbols in fantasy epics like Game of Thrones and The Witcher after going extinct around 12,000 years ago, but that’s all changed thanks to Colossal Biosciences. Colossal, who recently revealed the first Woolly Mouse, has now revealed it has brought the Dire Wolves back from extinction, introducing the world to three wolves named Remus, Romulus, and Khaleesi, and you can meet them in the video below.

The process of bringing the Dire Wolves back started with two DNA extractions, which came from a 13,000-year-old tooth and a 72,000-year-old inner ear bone. 20 edits were made across 14 genes, with 15 edits pulled directly from ancient DNA, and 5 edits informed from analysis from the ancient DNA, where the team chose what was best for the animal’s welfare. These ancient DNA variants haven’t been present for over 10,000 years.

Play video

Romulus and Remus were born in the first litter on October 1st, 2024, while Khaleesi was born in the second litter on January 30th, 2025. The wolves live on a secure and expansive ecological preserve that contains over 2000 acres, and if you’re wondering, yes, they have already met Game of Thrones creator George R.R. Martin, and even sat on the Iron Throne.